How to Use the Nanodrop Spectrophotometer

Nanodrop spectrophotometer is used to measure DNA or protein concentration or purity.

EditSteps

1. 1
   The Nanodrop machine and computer are switched on. Open the nanodrop program.
2. 2
   Add water to the point where the liquids are to be added. Click ok on the pop up window. This will initialize the software. Blot dry the surface with a blotting paper.
3. 3
   Add the control liquid, eg Nuclease free water. Click blank on the software window. This will set the software values , taring these values as zero.
4. 4
   Blot the surface of the interface again and add DNA. Click measure. The various parameters and OD ratio 360/380 ratio will come. For pure DNA, it should be 1.8. Protein contamination lowers it and RNA contamination elevates it.
5. I have written this article on wikihow. Hence, it is not copy-paste material.